Single Cell Whole Transcriptome Kit
Founded on the principles of the academic method SPLiT-seq (Science, 2018), Split Bio’s Whole Transcriptome kit provides the most scalable single cell RNA-seq solution on the market. Dramatic increases in transcript detection from the original academic method allow you to capture more information with less sequencing while maintaining low doublet rates.
up to 100,000 cells
up to 48 samples
The Split Bio Whole Transcriptome solution takes you from your single cell/nuclei suspension all the way to processed data. The workflow can be broken up into the following components:
Fixation: After sample extraction, take your single cell or single nuclei suspension(s) and perform a 30-60 minute protocol. You can store your samples for processing on a later date or proceed directly to cell barcoding.
Cell Barcoding: Load your fixed samples (1-48) and pass cells through four split-pool combinatorial barcoding steps to append a cell-specific combination of DNA barcodes to each transcript.
Library Prep: Take your barcoded molecules and prepare them for next generation sequencing. Here, you can select a subset of cells (i.e. 100-500 cells) to sequence for gene detection evaluation before continuing to sequence the rest of your cells.
Data Analysis: After sequencing, use our computational pipeline to output an experiment overview report with quality control metrics along with processed data that seamlessly integrates into existing open source post-processing tools.
Split Bio is accepting a limited number of research groups to enroll as part of an early access program. Participants will be able to purchase a single cell whole transcriptome kit and receive support from the Split Bio team. Let us know if you are interested and want to learn more.