Kits

The Split Bio Single Cell Whole Transcriptome Kit

 

100,000 cells and 48 samples with a single kit

Split Bio’s Whole Transcriptome kit is the most scalable single-cell RNA sequencing solution on the market. 

 

Dramatic increases in transcript detection from the original SPLiT-seq method allow you to capture more information with less sequencing while avoiding doublets. Click below to learn more details or sign up for our Early Access Program. 

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Split Bio Workflow

1

Fixation

Lock in gene expression immediately after tissue dissociation with a rapid fixation protocol. After fixation your samples can be stored for up to 6 months or you can proceed directly to barcoding.

2

Barcoding

Starting with 1-48 fixed biological samples, pass cells through three split-pool combinatorial barcoding steps to append a cell-specific combination of DNA barcodes to each transcript.

3

Library Prep

Prepare barcoded molecules for next generation sequencing. Up to 8 sequencing sublibraries (each containing 100-12,500 cells) can be prepared in parallel. Sublibraries can be sequenced independently at different sequencing depths. 

4

Data Analysis

After sequencing, use our computational pipeline to generate an experimental report along with processed data (including gene-cell count matrix) that integrates into existing open source tools (Seurat, Scanpy, etc.)

 
 
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Early Access Program

Split Bio is accepting a limited number of research groups to enroll as part of an Early Access Program. Participants will be able to purchase a Single Cell Whole Transcriptome kit and receive support from the Split Bio team. Let us know if you are interested and want to learn more.

PRODUCTS

info@splitbio.com

 

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Suite 304

Seattle, Washington 98195

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