Single Cell Whole Transcriptome
100,000 cells and 48 samples with a single kit
Based on the principles of the academic method SPLiT-seq, Split Bio’s Whole Transcriptome kit provides the most scalable single cell RNA-seq solution on the market. Dramatic increases in transcript detection from the original academic method allow you to capture more information with less sequencing while maintaining low doublet rates.
Split Bio is accepting a limited number of research groups to enroll as part of an early access program. Participants will be able to purchase a single cell whole transcriptome kit and receive support from the Split Bio team. Let us know if you are interested and want to learn more.
Following sample extraction, fix your single cell or single nuclei suspension(s) with a 30-60 minute protocol. After fixation your samples can be stored for up to 6 months or you can proceed directly to cell barcoding.
Load your fixed samples (1-48) and pass cells through four split-pool combinatorial barcoding steps to append a cell-specific combination of DNA barcodes to each transcript.
Prepare barcoded molecules for next generation sequencing. Up to 8 sequencing libraries (each containing 100-12,500 cells) can be prepared in parallel. Libraries can be sequenced independently at different sequencing depths.
After sequencing, use our computational pipeline to generate an experimental report along with processed data (including gene-cell count matrix) that integrates into existing open source tools (Seurat, Scanpy, etc.).