Split Bio Kit Workflow
Following extraction, fix your sample(s) with a 30-60 minute protocol. We offer both cell and nuclei fixation kits to accommodate your particular experiment. After fixation your samples can be stored for up to 6 months or you can proceed directly to barcoding.
Starting with 1-48 fixed biological samples, pass cells through four split-pool combinatorial barcoding steps to append a cell-specific combination of DNA barcodes to each transcript.
Prepare barcoded molecules for next generation sequencing. Up to 8 sequencing sublibraries (each containing 100-12,500 cells) can be prepared in parallel. Sublibraries can be sequenced independently at different sequencing depths.
After sequencing, use our computational pipeline to generate an experimental report along with processed data (including gene-cell count matrix) that integrates into existing open source tools (Seurat, Scanpy, etc.)