Split Bio Kit Workflow

Abstract Bubbles

1

Fixation

Following extraction, fix your sample(s) with a 30-60 minute protocol. We offer both cell and nuclei fixation kits to accommodate your particular experiment. After fixation your samples can be stored for up to 6 months or you can proceed directly to barcoding.

2

Barcoding

Starting with 1-48 fixed biological samples, pass cells through four split-pool combinatorial barcoding steps to append a cell-specific combination of DNA barcodes to each transcript.

3

Library Prep

Prepare barcoded molecules for next generation sequencing. Up to 8 sequencing sublibraries (each containing 100-12,500 cells) can be prepared in parallel. Sublibraries can be sequenced independently at different sequencing depths. 

4

Data Analysis

After sequencing, use our computational pipeline to generate an experimental report along with processed data (including gene-cell count matrix) that integrates into existing open source tools (Seurat, Scanpy, etc.)

PRODUCTS

info@splitbio.com

 

4000 Mason Rd

Suite 304

Seattle, Washington 98195

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